Development and Validation of Reversed-Phase HPLC Method for Simultaneous Estimation of Rosuvastatin and Fenofibrate in Tablet Dosage Form

A simple, precise and accurate reversed-phase liquid chromatographic method has been developed for the simultaneous estimation of Rosuvastatin and Fenofibrate in tablet formulations. The chromatographic separation was achieved on (Inertsil ODS, 250 x 4.6mm, 5μ column) analytical column. And mobile phase as mixture of Water (pH adjusted to 2.5 with ortho phosphoric acid) and acetonitrile in ratio (30:70 v/v) at flow rate of 1.0ml/min and dual detector wavelength 248 nm for Rosuvastatin and 286 nm for Fenofibrate. The retention time of Rosuvastatin and Fenofibrate was found to be 3.6 and 20.5 minutes respectively. The validation of the proposed method was carried out for its specificity, linearity, accuracy, precision, limit of detection and quantification for both atorvastatin calcium and telmisartan. The developed method can be used for routine quality analysis of titled drugs in combination in tablet formulation.